Bioinformatics DNA methods Bacterial genomic DNA extraction Bacterial genomic DNA extraction using CTAB DNA/RNA precipitation DNA digestion with Restriction Endonucleases DNA blunting Blunt end DNA cloning Colony PCR Gel staining with methylene blue Electrotransformation of E. coli Electrotransformation of plasmids Electrotransformation of P. aeruginosa Chromatin Immunoprecipitation Protein methods Coloração de proteínas com Coomassie G-250 Ensaio de catalase Western-blot Other Methods ppGpp assay